| Gene name |
SPCC24B10.07 |
| Gene ID |
45/G11 |
| Gene synonyms/obsolete |
|
| Gene product |
serine/threonine
protein kinase; AGC family; TOR signaling pathway;
non-essential; involved in conjugation (required); involved in
the regulation of G1 progression; phosphorylated by Tor1p; C2
domain (SMART) |
| Entry clone |
Cloned in 2004
trial |
| ORF length (unspliced) |
1781 |
| ORF length (spliced) |
1710 |
| Entry clone length |
1781 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC24B10.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCTGGAAACTTACAAA |
| Rev primer name |
SPCC24B10.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCTAATGACACTTCCAGGT |
| Amino acid length |
569 |
| Molecular weight |
63.7 |
| Isoelectric point (calc.) |
8.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |