| Gene name |
SPBC211.06 |
| Gene ID |
45/F10 |
| Gene synonyms/obsolete |
|
| Gene product |
gamma tubulin complex
subunit; non-essential; GRIP domain; similar to human GPC4; no
apparent S. cerevisiae ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1734 |
| ORF length (spliced) |
|
| Entry clone length |
1734 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC211.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGCATGAGATTATTTT |
| Rev primer name |
SPBC211.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAAATTAAGCTGAACAATC |
| Amino acid length |
577 |
| Molecular weight |
66.7 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRLLLSKKLFL/LRKTISLLDL/LWILLGSLHL/LNSIYHELSL |
| Localization (YFP) |
nucleus>>cytosol; SPB |
| Comments for localization |
nuclear dots by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |