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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC12G12.03
Gene ID 45/F08
Gene synonyms/obsolete
Gene product conserved hypothetical; RNA-binding protein; rrm RNA recognition motif
Entry clone Cloned
ORF length (unspliced) 1731
ORF length (spliced)
Entry clone length 1731
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC12G12.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTGAGGGCACAAGCCA
Rev primer name SPAC12G12.03.Rv
Rev primer SEQ AGAAAGCTGGGTAATTCTTTGTAAAAGTTCTG
Amino acid length 576
Molecular weight 62
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFKVFTALDI
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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