| Gene name |
SPCC1259.09c |
| Gene ID |
45/D10 |
| Gene synonyms/obsolete |
|
| Gene product |
pyruvate dehydrogenase
protein x component; involved in acetyl-CoA biosynthesis from
pyruvate; biotin/lipoyl attachment site; e3 binding
domain |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1627 |
| ORF length (spliced) |
1371 |
| Entry clone length |
1627 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
#check |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1259.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGAAGCATTACATTCA |
| Rev primer name |
SPCC1259.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCAGCGCGATAAGACAAAT |
| Amino acid length |
456 |
| Molecular weight |
50.8 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |