| Gene name |
SPAC1B1.02c |
| Gene ID |
45/D04 |
| Gene synonyms/obsolete |
|
| Gene product |
NAD kinase; ATP
dependent; involved in nicotinate and nicotinamide metabolism;
similar to Sp SPAC3H5.11 and SPCC24B10.02C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1614 |
| ORF length (spliced) |
|
| Entry clone length |
1614 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC1B1.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACCGGGCTCGAAACT |
| Rev primer name |
SPAC1B1.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTTGCTCTTCGGAAGTA |
| Amino acid length |
537 |
| Molecular weight |
60.7 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTKKLSSLSI/LAFRFRKVLLI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol) |
| Microscope used for
observation |
Leica |