| Gene name |
SPCC1450.15 |
| Gene ID |
45/B01 |
| Gene synonyms/obsolete |
|
| Gene product |
phosphoethanolamine
N-methyltransferase activity; involved in GPI anchor
biosynthesis; similar to human PIG-F |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1562 |
| ORF length (spliced) |
1512 |
| Entry clone length |
1562 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1450.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTTGTACGTTTATCAA |
| Rev primer name |
SPCC1450.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGAAGAATCTCTCCAACT |
| Amino acid length |
503 |
| Molecular weight |
56.6 |
| Isoelectric point (calc.) |
7.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
5 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGKAIAKELVL/LVGSLLSSLPI/LLLTFTQLTI/LTYFCALTL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |