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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F5.07c
Gene ID 44/G07
Gene synonyms/obsolete
Gene product protoporphyrinogen oxidase
Entry clone Cloned
ORF length (unspliced) 1473
ORF length (spliced)
Entry clone length 1473
No. of intron 0
Sequence status Finished
Sequence results 535G:A
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1F5.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAATTGCAATTTGTGG
Rev primer name SPAC1F5.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAGAAGAAGAATTCATCAAT
Amino acid length 490
Molecular weight 53.4
Isoelectric point (calc.) 9.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDLISKLGI/LKALQHTLKI
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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