| Gene name |
SPAC31A2.09c |
| Gene ID |
44/F07 |
| Gene synonyms/obsolete |
apm4 |
| Gene product |
clathrin coat assembly
protein; AP adaptor complex; involved in vesicle-mediated
protein transport |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1437 |
| ORF length (spliced) |
1341 |
| Entry clone length |
1437 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC31A2.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTTCGGTTAGTATTAA |
| Rev primer name |
SPAC31A2.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATGCGTATCTCGCAAGTT |
| Amino acid length |
446 |
| Molecular weight |
50.8 |
| Isoelectric point (calc.) |
9.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB; spindle
microtubules; periphery at site of septum formation;
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |