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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC622.14
Gene ID 44/F04
Gene synonyms/obsolete
Gene product zinc finger protein; zf-GCS type; putative ADP-ribosylation factor; GTPase-activating protein
Entry clone Cloned
ORF length (unspliced) 1425
ORF length (spliced) 930
Entry clone length 1425
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC622.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGTATAAATTAGACCA
Rev primer name SPCC622.14.Rv
Rev primer SEQ AGAAAGCTGGGTAATCGTGCTTATCGAAATTC
Amino acid length 309
Molecular weight 34.1
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots, especially at cell tip and site of septum formation
Comments for localization accumulated localization by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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