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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC29A4.15
Gene ID 44/C09
Gene synonyms/obsolete
Gene product serine-tRNA ligase; involved in seryl-tRNA aminoacylation; serine-tRNA ligase activity
Entry clone Cloned
ORF length (unspliced) 1353
ORF length (spliced)
Entry clone length 1353
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC29A4.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTAGACATAAACTTGTT
Rev primer name SPAC29A4.15.Rv
Rev primer SEQ AGAAAGCTGGGTAATTCTTTCCCTTCTTGGAG
Amino acid length 450
Molecular weight 51.6
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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