| Gene name |
SPCC1259.10 |
| Gene ID |
44/C07 |
| Gene synonyms/obsolete |
|
| Gene product |
peptidase family M22;
glycoprotease; zinc metalloprotease; endopeptidase;
overexpression rescues krp1 mutant |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1332 |
| ORF length (spliced) |
1239 |
| Entry clone length |
1332 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1259.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTTGTCTGGTATATAA |
| Rev primer name |
SPCC1259.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACTTTTTTATTTTTTTTT |
| Amino acid length |
412 |
| Molecular weight |
45.9 |
| Isoelectric point (calc.) |
9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
11 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLALKRLDL |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |