Gene name |
SPAC343.14c |
Gene ID |
44/B07 |
Gene synonyms/obsolete |
|
Gene product |
translation initiation
factor eif-2b (beta subunit) |
Entry clone |
Cloned |
ORF length (unspliced) |
1280 |
ORF length (spliced) |
1182 |
Entry clone length |
1280 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC343.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAACTATTAATGTTGA |
Rev primer name |
SPAC343.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGAATTGTATCGCTTGCA |
Amino acid length |
393 |
Molecular weight |
43.2 |
Isoelectric point (calc.) |
6.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
217 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |