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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC458.04c
Gene ID 43/G08
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1110
ORF length (spliced) 1023
Entry clone length 1110
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC458.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACGAGCACGGATGTTT
Rev primer name SPAC458.04.Rv
Rev primer SEQ AGAAAGCTGGGTATTGAATTAAGTCCTTCCAC
Amino acid length 340
Molecular weight 38.8
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVRLTNYLML/LKQLKHALGL
Localization (YFP) cytosol
Comments for localization faint ambiguous structure
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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