Gene name |
SPAC22F3.02 |
Gene ID |
42/E08 |
Gene synonyms/obsolete |
atf31 |
Gene product |
putative transcription
factorbZIP (basic leucine zipper) transcription factor family;
overexpression suppresses calcium sensitivity of Atf1
deletants; non-essential; involved in regulation of meiosis;
involved in regulation of transcription from PolII promoter;
transcription factor activity; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
630 |
ORF length (spliced) |
|
Entry clone length |
630 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC22F3.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACCTACGAAACAAATAC |
Rev primer name |
SPAC22F3.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGGAGGCGTAAGCGGTTTT |
Amino acid length |
209 |
Molecular weight |
23.9 |
Isoelectric point (calc.) |
6.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus; nuclear
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |