| Gene name |
SPAPB1A10.04c |
| Gene ID |
41/G11 |
| Gene synonyms/obsolete |
cwp1 |
| Gene product |
geranylgeranyltransferase type I (GGTase I), alpha
subunit; involved in the post-translational C-terminal
modification of several small GTPases, allowing their
targeting to the membrane; essential |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
936 |
| ORF length (spliced) |
885 |
| Entry clone length |
936 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAPB1A10.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATCCCATTGATCCCGA |
| Rev primer name |
SPAPB1A10.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGATTGCTCCTTTGGGAC |
| Amino acid length |
294 |
| Molecular weight |
34.8 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |