| Gene name |
SPBC32H8.03 |
| Gene ID |
41/G05 |
| Gene synonyms/obsolete |
bem46;
SPACTOKYO_453.15c |
| Gene product |
suppressor of bem1;
suppressor of bud5 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
900 |
| ORF length (spliced) |
|
| Entry clone length |
900 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
6A:G / 292A:G /
777T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC32H8.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGGTTCATTGTCTTC |
| Rev primer name |
SPBC32H8.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGAAGCAGGTGTGTTGATA |
| Amino acid length |
299 |
| Molecular weight |
33.1 |
| Isoelectric point (calc.) |
8.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |