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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1322.11
Gene ID 41/E03
Gene synonyms/obsolete rpl2302; rpl23-2
Gene product 60S ribosomal protein L23
Entry clone Cloned
ORF length (unspliced) 685
ORF length (spliced) 420
Entry clone length 685
No. of intron 1
Sequence status Finished
Sequence results 179A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1322.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTCGTGGACGCGGTGC
Rev primer name SPCC1322.11.Rv
Rev primer SEQ AGAAAGCTGGGTAAACAACGGTACCAGCATTT
Amino acid length 139
Molecular weight 14.8
Isoelectric point (calc.) 10.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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