| Gene name |
SPBC776.02c |
| Gene ID |
40/E03 |
| Gene synonyms/obsolete |
bws1; dis2; sds1 |
| Gene product |
serine/threonine
protein phosphatase pp1-1; involved in sister chromatid
separation (implicated) (mitosis); required for chromosome
disjoining |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1380 |
| ORF length (spliced) |
984 |
| Entry clone length |
1380 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1119T:C /
1192T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC776.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGAACCCAGATGTGGA |
| Rev primer name |
SPBC776.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTTGAATTTCCTGTCTTA |
| Amino acid length |
327 |
| Molecular weight |
37.6 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLELEAPLKI |
| Localization (YFP) |
nucleus>cytosol;
cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |