| Gene name |
SPBC17F3.02 |
| Gene ID |
39/F06 |
| Gene synonyms/obsolete |
nak1 |
| Gene product |
N-terminal
serine/threonine protein kinase; involved in cellular
morphogenesis (regulation); essential; involved in cell growth
(regulation); PAK-related kinase Nak1; GCK subfamily;
essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2075 |
| ORF length (spliced) |
1959 |
| Entry clone length |
2075 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC17F3.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAATAACACTGCTTC |
| Rev primer name |
SPBC17F3.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCATTATATGCTTTTCTG |
| Amino acid length |
652 |
| Molecular weight |
71.1 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRAHLPPLSI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |