| Gene name |
SPBC660.16 |
| Gene ID |
38/H12 |
| Gene synonyms/obsolete |
|
| Gene product |
phosphogluconate
dehydrogenase, decarboxylating; involved in pentose-phosphate
shunt, oxidative branch; involved in glucose metabolism;
phosphogluconate dehydrogenase (decarboxylating)
activity |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
2694 |
| ORF length (spliced) |
1479 |
| Entry clone length |
2694 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC660.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCACAAAAAGAAGTGTA |
| Rev primer name |
SPBC660.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCATCATAGGTAGTAGCA |
| Amino acid length |
492 |
| Molecular weight |
53.6 |
| Isoelectric point (calc.) |
7.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAVMGQNLIL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |