| Gene name |
SPBC582.11c |
| Gene ID |
38/H03 |
| Gene synonyms/obsolete |
nup107;
SPBC428.01c |
| Gene product |
nucleoporin; nuclear
pore complex (putative); essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2668 |
| ORF length (spliced) |
2343 |
| Entry clone length |
2668 |
| No. of intron |
6 |
| Sequence status |
Finished |
| Sequence results |
1697G:A /
2218T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC582.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCTTGGACACAAAAAA |
| Rev primer name |
SPBC582.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAAAGAAAAAAGCCCATTA |
| Amino acid length |
780 |
| Molecular weight |
89.7 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPPVDSGLGL/LTHIILFLKI/LICTLEWLLI |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |