Gene name |
SPAC20H4.09 |
Gene ID |
38/B07 |
Gene synonyms/obsolete |
SPAC145.03 |
Gene product |
DEAD/DEAH box
helicase; ATP-dependent; RNA helicase; involved in mRNA
splicing |
Entry clone |
Cloned |
ORF length (unspliced) |
2293 |
ORF length (spliced) |
1944 |
Entry clone length |
2293 |
No. of intron |
8 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC145.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTAATGAGTTTATTTC |
Rev primer name |
SPAC145.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTTCTCGTTTATAGTAT |
Amino acid length |
647 |
Molecular weight |
73 |
Isoelectric point (calc.) |
7.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFPVETLFL/LNCDMSPLVL/LMAALEDLYL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |