| Gene name |
SPAC630.05 |
| Gene ID |
38/A11 |
| Gene synonyms/obsolete |
|
| Gene product |
TBC domain protein;
GTPase activating protein of Rab-like GTPase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2232 |
| ORF length (spliced) |
|
| Entry clone length |
2232 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
110A:T / 1111A:G /
2214G:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC630.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACAACGCTGGAATCTCT |
| Rev primer name |
SPAC630.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTTGCTTACCACTCGGC |
| Amino acid length |
743 |
| Molecular weight |
86.7 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQVDVHRVLGI/LFCFFRMLLI |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |