| Gene name |
SPBC30B4.02c |
| Gene ID |
37/C11 |
| Gene synonyms/obsolete |
|
| Gene product |
R3H domain; G-patch
domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2088 |
| ORF length (spliced) |
|
| Entry clone length |
2088 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
486T:C / 1199A:G /
1315A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC30B4.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAATTTTGATGATCT |
| Rev primer name |
SPBC30B4.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACCTAAACCACTTCTGCTA |
| Amino acid length |
695 |
| Molecular weight |
79 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
420/419 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVHALANDLNL |
| Localization (YFP) |
cytosol; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus; cytoplasmic dots |
| Microscope used for
observation |
Leica |