| Gene name |
SPBC354.07c |
| Gene ID |
36/G06 |
| Gene synonyms/obsolete |
|
| Gene product |
oxysterol binding
protein; involved in ergosterol biosynthesis; similar to Sp
SPBC646.08c and SPBC1271.12 and SPCC23B6.01 |
| Entry clone |
Cloned/2003.08 |
| ORF length (unspliced) |
1836 |
| ORF length (spliced) |
1200 |
| Entry clone length |
1836 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC354.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATAGGAGCACTTGGATG |
| Rev primer name |
SPBC354.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCCATCCGTTTGACAGGT |
| Amino acid length |
399 |
| Molecular weight |
45.5 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
10 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKSTIFFLSI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |