| Gene name |
SPCC18B5.11c |
| Gene ID |
36/F11 |
| Gene synonyms/obsolete |
cds1 |
| Gene product |
serine/threonine
protein kinase; non-essential; involved in DNA damage
checkpoint; involved in DNA replication checkpoint; FHA domain
(phosphopeptide binding) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1829 |
| ORF length (spliced) |
1383 |
| Entry clone length |
1829 |
| No. of intron |
7 |
| Sequence status |
Finished |
| Sequence results |
359A:G / 860A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC18B5.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGAACCAGAAGAAGC |
| Rev primer name |
SPCC18B5.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACTCGAAGAATTGAGCTGT |
| Amino acid length |
460 |
| Molecular weight |
52 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKQLLETLLHL |
| Localization (YFP) |
nucleus>>cytosol; site of septum
formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |