| Gene name |
SPCC1906.02c |
| Gene ID |
36/A02 |
| Gene synonyms/obsolete |
|
| Gene product |
role inferred from
homology; CUE domain protein; involved in the ubiquitin
system |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1746 |
| ORF length (spliced) |
|
| Entry clone length |
1746 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
14T:G / 958T:C /
981T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1906.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGAGAATGTCATATC |
| Rev primer name |
SPCC1906.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTCTCATTTGAAGCCTTT |
| Amino acid length |
581 |
| Molecular weight |
65.8 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEMLLDSLDL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol) |
| Microscope used for
observation |
Leica |