Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F7.02c
Gene ID 35/H01
Gene synonyms/obsolete
Gene product DEAD/DEAH box helicase; ATP-dependent; RNA helicase
Entry clone Cloned
ORF length (unspliced) 1737
ORF length (spliced)
Entry clone length 1737
No. of intron 0
Sequence status Finished
Sequence results 840T:C / 1278T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1F7.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTAAATCCGAGTTAAA
Rev primer name SPAC1F7.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGCGGCTCCATCCACGCTCT
Amino acid length 578
Molecular weight 65.2
Isoelectric point (calc.) 9.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>>nucleus
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleolus>nucleus)
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images
LMB 4 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.