Gene name |
SPCC338.16 |
Gene ID |
35/G10 |
Gene synonyms/obsolete |
pof3 |
Gene product |
F-box protein; TPR
repeat protein; involved in genome integrity (required) and
telomere maintenance (required) |
Entry clone |
Cloned |
ORF length (unspliced) |
1734 |
ORF length (spliced) |
|
Entry clone length |
1734 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
505T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC338.16.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATAACTATCAAGTAAA |
Rev primer name |
SPCC338.16.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATATTAGGCGTATCTTTTTT |
Amino acid length |
577 |
Molecular weight |
66.2 |
Isoelectric point (calc.) |
9.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LINLELFLYL/LPELEHLEI |
Localization (YFP) |
mitochondrion;
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |