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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC36.02c
Gene ID 35/G07
Gene synonyms/obsolete
Gene product unknown specificity; transporter; similar to Sp SPAC11D3.05 and SPCC794.04C and SPCC569.05C and SPBC36.03C and SPBC36.01C and SPBC530.15C and SPBC530.02 and SPBC947.06C; involved in amine/polyamine transport; tandem duplication
Entry clone Cloned
ORF length (unspliced) 1734
ORF length (spliced)
Entry clone length 1734
No. of intron 0
Sequence status Finished
Sequence results 626T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC36.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTCAACTCCTACGGC
Rev primer name SPBC36.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAATCGACGGCCATCTTG
Amino acid length 577
Molecular weight 63.6
Isoelectric point (calc.) 8.1
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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