Gene name |
SPBC1A4.08c |
Gene ID |
35/D01 |
Gene synonyms/obsolete |
cct3 |
Gene product |
chaperonin-containing
T-complex; T-complex protein 1 (gamma subunit); involved in
protein folding; involved in actin folding and tubulin
folding |
Entry clone |
Cloned |
ORF length (unspliced) |
1695 |
ORF length (spliced) |
1587 |
Entry clone length |
1695 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
240G:A / 1077T:C /
1495G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1A4.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGTCTCCTGTGTTTGT |
Rev primer name |
SPBC1A4.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCGGAGTGCTTGCGTACA |
Amino acid length |
528 |
Molecular weight |
58.4 |
Isoelectric point (calc.) |
7.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |