| Gene name |
SPCC1840.08c |
| Gene ID |
35/C04 |
| Gene synonyms/obsolete |
|
| Gene product |
glycoprotein;
thioredoxin related glycoprotein; similar to Sp SPAC959.05C
and SPBC3D6.13C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1686 |
| ORF length (spliced) |
|
| Entry clone length |
1686 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1840.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGCTGGGTGCTTGGAG |
| Rev primer name |
SPCC1840.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCTTGCTTTAACAAAGAG |
| Amino acid length |
561 |
| Molecular weight |
64.7 |
| Isoelectric point (calc.) |
9.5 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDTTFRVLAI/LISLWISLIL |
| Localization (YFP) |
ER; Golgi |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |