| Gene name |
SPAC824.08 |
| Gene ID |
34/H12 |
| Gene synonyms/obsolete |
gdp1 |
| Gene product |
guanosine-diphosphatase Gda1; similar to S.
cerevisiae YEL042W; 1 predicted transmembrane helix;
predicted N-terminal signal sequence; double mutant
gda1delta/ynd1delta lethal |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1671 |
| ORF length (spliced) |
|
| Entry clone length |
1671 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC824.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTCCAACTATGAAATC |
| Rev primer name |
SPAC824.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATTTCTTCTTTCACGTTA |
| Amino acid length |
556 |
| Molecular weight |
61.5 |
| Isoelectric point (calc.) |
4.6 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER; Golgi |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |