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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC18G6.05c
Gene ID 34/D03
Gene synonyms/obsolete
Gene product HEAT repeat; involved in translational activation
Entry clone Cloned
ORF length (unspliced) 8086
ORF length (spliced) 8013
Entry clone length 8086
No. of intron 1
Sequence status Finished
Sequence results 2121T:C / 5673T:C / 6950A:G / 7349G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC18G6.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGGTCGAAGAACCTGG
Rev primer name SPAC18G6.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGATCATTAGTGGAAACT
Amino acid length 2670
Molecular weight 297.3
Isoelectric point (calc.) 5.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LQYWILELLPL/LYHEFCYSLGL/LLSVDQLKL/LIKSLLHLLEI/LPDLLPLLL/LSAFGVKLLL/LSEILAGLGL/LESKVLSLEI
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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