| Gene name |
SPAC18G6.05c |
| Gene ID |
34/D03 |
| Gene synonyms/obsolete |
|
| Gene product |
HEAT repeat; involved
in translational activation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
8086 |
| ORF length (spliced) |
8013 |
| Entry clone length |
8086 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
2121T:C / 5673T:C /
6950A:G / 7349G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC18G6.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGGTCGAAGAACCTGG |
| Rev primer name |
SPAC18G6.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGATCATTAGTGGAAACT |
| Amino acid length |
2670 |
| Molecular weight |
297.3 |
| Isoelectric point (calc.) |
5.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQYWILELLPL/LYHEFCYSLGL/LLSVDQLKL/LIKSLLHLLEI/LPDLLPLLL/LSAFGVKLLL/LSEILAGLGL/LESKVLSLEI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |