Gene name |
SPBC8D2.10c |
Gene ID |
33/F05 |
Gene synonyms/obsolete |
|
Gene product |
zinc finger protein
(inferred from context); zf-C2H2 type; arginine
N-methyltransferase; no apparent Sc ortholog (has
homologs) |
Entry clone |
Cloned in 2004
trial |
ORF length (unspliced) |
2025 |
ORF length (spliced) |
1632 |
Entry clone length |
2025 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC8D2.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTAGTGAAACCTATGGC |
Rev primer name |
SPBC8D2.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTCAACACGTAGCTTTGA |
Amino acid length |
543 |
Molecular weight |
61.7 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
Microscope used for
observation |
DeltaVision |