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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1347.10
Gene ID 33/D07
Gene synonyms/obsolete cdc23
Gene product MCM-associated protein; involved in DNA replication (initiation) (required); essential; OB-fold nucleic acid binding domain; interacts physically with Mcm4p; interacts physically with Mcm5p; interacts physically with Mcm6p; interacts physically with Orc1p; interacts physically with Orc2p; interacts physically with Orc5p; interacts physically with Orc6p
Entry clone Cloned
ORF length (unspliced) 1782
ORF length (spliced)
Entry clone length 1782
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1347.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCATGATCCCTTCATTGC
Rev primer name SPBC1347.10.Rv
Rev primer SEQ AGAAAGCTGGGTAGGGAACTATTTCTAAGTCA
Amino acid length 593
Molecular weight 66.6
Isoelectric point (calc.) 9.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) spindle microtubules; nucleus; bright nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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