| Gene name |
SPAC637.05c |
| Gene ID |
33/D05 |
| Gene synonyms/obsolete |
vma2 |
| Gene product |
V-type ATPase; ATP
synthase (catalytic subunit B); localization vacuole |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1749 |
| ORF length (spliced) |
1512 |
| Entry clone length |
1749 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
137T:C / 1387A:G /
1433A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC637.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGTCGCTCTTTGATAT |
| Rev primer name |
SPAC637.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGAGCTTTCAGGAGTGTTG |
| Amino acid length |
503 |
| Molecular weight |
55.8 |
| Isoelectric point (calc.) |
4.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
cytoplasmic dots by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |