Gene name |
SPBC1734.15 |
Gene ID |
33/D03 |
Gene synonyms/obsolete |
brd1 |
Gene product |
involved in
transcriptional regulation; involved in chromatin remodeling;
bromodomain protein; BHA domain |
Entry clone |
Cloned |
ORF length (unspliced) |
1737 |
ORF length (spliced) |
1629 |
Entry clone length |
1737 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
416T:C / 737T:C /
1121A:G / 1715T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1734.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTATATTCTCATGTCCC |
Rev primer name |
SPBC1734.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGGTTGGACGTAAGCCATC |
Amino acid length |
542 |
Molecular weight |
60.6 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |