| Gene name |
SPBC1861.02 |
| Gene ID |
33/C03 |
| Gene synonyms/obsolete |
abp2 |
| Gene product |
ARS binding protein;
AT hook protein; non-essential; deletion mutant results in
nuclear morphology defects; deletion mutant sensitive to UV;
deletion results in aberrant septa; no apparent
orthologs |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1584 |
| ORF length (spliced) |
|
| Entry clone length |
1584 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
430A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1861.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACTTTTATTCCTTGTT |
| Rev primer name |
SPBC1861.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTCAAACACTGCATTCATA |
| Amino acid length |
527 |
| Molecular weight |
59.6 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDTVSSLKL |
| Localization (YFP) |
SPB; nuclear dots;
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |