Gene name |
SPBC17A3.07 |
Gene ID |
33/A07 |
Gene synonyms/obsolete |
pgr1; pi039 |
Gene product |
glutathione reductase;
essential; maintains high levels of glutathione in the cytosol
|
Entry clone |
Cloned |
ORF length (unspliced) |
1450 |
ORF length (spliced) |
1395 |
Entry clone length |
1450 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
1251A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC17A3.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCACCTATTTCAAAGGT |
Rev primer name |
SPBC17A3.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACTAAAGTAACTAGTTCT |
Amino acid length |
464 |
Molecular weight |
49.9 |
Isoelectric point (calc.) |
7.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGDVCGKLEL/LQKVVGLHL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |