| Gene name |
SPBC17A3.07 |
| Gene ID |
33/A07 |
| Gene synonyms/obsolete |
pgr1; pi039 |
| Gene product |
glutathione reductase;
essential; maintains high levels of glutathione in the cytosol
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
1450 |
| ORF length (spliced) |
1395 |
| Entry clone length |
1450 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1251A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC17A3.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCACCTATTTCAAAGGT |
| Rev primer name |
SPBC17A3.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACTAAAGTAACTAGTTCT |
| Amino acid length |
464 |
| Molecular weight |
49.9 |
| Isoelectric point (calc.) |
7.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGDVCGKLEL/LQKVVGLHL |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |