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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1711.13
Gene ID 32/H01
Gene synonyms/obsolete his2
Gene product histidinol dehydrogenase
Entry clone Cloned
ORF length (unspliced) 1320
ORF length (spliced)
Entry clone length 1320
No. of intron 0
Sequence status Finished
Sequence results 713A:G / 722C:T / 860T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1711.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCGGAATACGAGATTCA
Rev primer name SPBC1711.13.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGTCTAACTCCCCGAACA
Amino acid length 439
Molecular weight 47.5
Isoelectric point (calc.) 7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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