| Gene name |
SPBC23G7.12c |
| Gene ID |
32/G02 |
| Gene synonyms/obsolete |
let1; rpt6 |
| Gene product |
19S proteasome
regulatory subunit; AAA family ATPase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1254 |
| ORF length (spliced) |
1212 |
| Entry clone length |
1254 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC23G7.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACGGAGGTGCTGAAGAC |
| Rev primer name |
SPBC23G7.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTGAAAAGCTTTTGTAAA |
| Amino acid length |
403 |
| Molecular weight |
45 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear envelope;
nucleus; cytosol |
| Comments for localization |
except nucleolus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |