| Gene name |
SPAPYUG7.04c |
| Gene ID |
31/G02 |
| Gene synonyms/obsolete |
rpb9 |
| Gene product |
DNA-directed RNA
polymerase II (subunit); involved in transcription from Pol II
promoter; DNA-directed RNA polymerase activity (function of
complex); |
| Entry clone |
Cloned |
| ORF length (unspliced) |
649 |
| ORF length (spliced) |
342 |
| Entry clone length |
649 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAPYUG7.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAATTTTCAATATTG |
| Rev primer name |
SPAPYUG7.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTGTTCTTCAAAAGCAAAT |
| Amino acid length |
113 |
| Molecular weight |
13.1 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |