| Gene name |
SPAC4F10.14c |
| Gene ID |
31/F10 |
| Gene synonyms/obsolete |
btf3 |
| Gene product |
nascent
polypeptide-associated complex; chaperone |
| Entry clone |
Cloned |
| ORF length (unspliced) |
614 |
| ORF length (spliced) |
456 |
| Entry clone length |
614 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
192T:C / 340T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4F10.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATCCCTCCAAATTGGC |
| Rev primer name |
SPAC4F10.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCTTGTTCGTCAAACTTT |
| Amino acid length |
151 |
| Molecular weight |
16.2 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
Leica |