| Gene name |
SPBC21C3.13 |
| Gene ID |
31/F02 |
| Gene synonyms/obsolete |
rps1901; rps19-1 |
| Gene product |
40S ribosomal protein
(S19.1/S19A subunit) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
591 |
| ORF length (spliced) |
435 |
| Entry clone length |
591 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
195A:G / 202T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC21C3.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGGCGTTTCTGTTAA |
| Rev primer name |
SPBC21C3.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCGCTTTCTTCTTCCAAA |
| Amino acid length |
144 |
| Molecular weight |
16.1 |
| Isoelectric point (calc.) |
10.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus>nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |