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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP16F5.05c
Gene ID 31/D09
Gene synonyms/obsolete
Gene product ankyrin repeat protein
Entry clone Cloned
ORF length (unspliced) 441
ORF length (spliced)
Entry clone length 441
No. of intron 0
Sequence status Finished
Sequence results 183A:G / 431A:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP16F5.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGTTGATGATTTAAT
Rev primer name SPBP16F5.05.Rv
Rev primer SEQ AGAAAGCTGGGTATATTCCATCTTCCAATTTT
Amino acid length 146
Molecular weight 16.2
Isoelectric point (calc.) 4.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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