| Gene name |
SPBC8D2.09c |
| Gene ID |
31/D05 |
| Gene synonyms/obsolete |
|
| Gene product |
U2 b'-like
spliceosomal protein; U2 snRNA-associated protein; involved in
mRNA splicing; rrm RNA recognition motif; RNA-binding
protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
409 |
| ORF length (spliced) |
336 |
| Entry clone length |
409 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
90T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC8D2.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATCAAAATACACTTTA |
| Rev primer name |
SPBC8D2.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCAAGGTCAGCTTCATCA |
| Amino acid length |
111 |
| Molecular weight |
12.6 |
| Isoelectric point (calc.) |
10.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |