| Gene name |
SPBC577.06c |
| Gene ID |
31/B11 |
| Gene synonyms/obsolete |
|
| Gene product |
phosphatidylinositol
4-kinase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
5822 |
| ORF length (spliced) |
5634 |
| Entry clone length |
5822 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
124T:C / 1558A:C /
1763A:G / 3377A:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC577.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTGGTAAGTGGTTTT |
| Rev primer name |
SPBC577.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATAAGGAATACCTTAAAAA |
| Amino acid length |
1877 |
| Molecular weight |
213.9 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRFYLLNYLFL/LTQFQSILNL/LFFVLVLKLAL/LFRDMWFTLVI/LPCFKGQLTI |
| Localization (YFP) |
cytosol |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |