Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F7.01c
Gene ID 30/F06
Gene synonyms/obsolete SPAC694.07c
Gene product involved in establishment and/or maintenance of chromatin architecture; SH2 domain; S1 domain; CSZ domain (SMART)
Entry clone Cloned
ORF length (unspliced) 4098
ORF length (spliced)
Entry clone length 4098
No. of intron 0
Sequence status Finished
Sequence results 48A:G / 314A:G / 844A:G / 1874A:G / 2066T:C / 3270C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1F7.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTGAAAACGAAGTTGT
Rev primer name SPAC1F7.01.Rv
Rev primer SEQ AGAAAGCTGGGTACATCCGTCTAAAATTTTTA
Amino acid length 1365
Molecular weight 157.2
Isoelectric point (calc.) 4.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 88/86/85
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDELIVLHI
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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