| Gene name |
SPCC645.07 |
| Gene ID |
30/E12 |
| Gene synonyms/obsolete |
rgf1 |
| Gene product |
non-essential; RhoGEF;
pleckstrin homology domain; similar to Sp SPCC645.06c and
SPAC1006.06 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4051 |
| ORF length (spliced) |
4005 |
| Entry clone length |
4051 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
209A:C / 291T:C /
1252T:C / 4020C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC645.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTACCGGCATCCAAA |
| Rev primer name |
SPCC645.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTGTCTACATGCTGCTCT |
| Amino acid length |
1334 |
| Molecular weight |
150.1 |
| Isoelectric point (calc.) |
9.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
406 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFLFDHALLI/LRIVKELYI |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |